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  • ABE1075 - Anti-WD repeat-containing protein 5 Antibody

ABE1075 Sigma-Aldrich

Anti-WD repeat-containing protein 5 Antibody

serum, clone, from rabbit

Synonym: WD repeat-containing protein 5, BMP2-induced 3-kb gene protein

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Primary Antibodies, W
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   human, rat, mouse
shipped in   wet ice
antibody form   serum
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_060058
UniProt accession no.   P61964


General description

WD repeat-containing protein 5 (WDR5) or alternatively BMP2-induced 3kb gene protein, is encoded by the human gene named WDR5/BIG3 and is part of a family of WD-40 repeat proteins that appear to facilitate the formation of multi-protein complexes much like a sticky tape will bind a variety of components together. The WD repeat proteins are involved in a wide array of cellular functions and processes including cell cycle progression, homeobox gene activation, apoptosis, gene regulation and histone methylation. One of WDR5 best studied activities is as part of a multi-protein complex that facilitates the methylation of histones, particularly histone H3, through its interaction with MLL a histone methyltransferase. WDR5 is also expressed in developing bone cells including osteoblasts, chondrocytes, and marrow stromal cells and works to accelerate osteoblast differentiation and positively promotes Wnt signaling in developing bone tissues.


Linear peptide corresponding to human WD repeat containing protein 5.


Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

This Anti-WD repeat-containing protein 5 antibody is validated for use in western blotting for the detection of WD repeat-containing protein 5.

Western Blotting Analysis: A representative lot from an independent laboratory detected WD repeat containing protein 5 in tested cell lysates. (Wysocka, J., et al. (2003). Genes Dev. 17(7):896-911.; Tyagi, S., et al. (2007). 27(1):107-119.; Jin, S., et al. (2010). J Clin Invest. 120(2):593-606.; Tyagi, S., et al. (2009). EMBO J. 28(20):3185-3195.; Diao, Y., et al. (2012). Cell Stem Cell. 11(2):231-241.; Schram, A. W., et al. (2013). PLoS One. 8(7):e70035.).

Target description

~37 kDa observed

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.


Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Evaluated by Western Blotting in NIH/3T3 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WD repeat containing protein 5 in 10 µg of NIH/3T3 cell lysate.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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