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ABE171 Sigma-Aldrich

Anti-MeCP2 Antibody

from chicken, purified by affinity chromatography

Synonym: methyl CpG binding protein 2 (Rett syndrome), mental retardation, X-linked 16, MeCp-2 protein, mental retardation, X-linked 79, methyl-CpG-binding protein 2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MB-ME, Primary Antibodies
clone   polyclonal
biological source   chicken
application(s)   ChIP: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, rat, mouse
shipped in   wet ice
isotype   IgY
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_004983
UniProt accession no.   P51608
Gene Information   human ... MECP2(4204)

Description

General description

MeCp2 (methyl-CpG-binding protein 2) binds methylated CpG sequences in double-stranded DNA resulting in the suppression of transcription. Although MeCp2 is not essential in stem cells, it is critical for embryonic development. This protein seems to be particularly important for mature nerve cells, where it is present in high levels. The MeCp2 protein is likely to be involved in turning off ("repressing" or "silencing") several other genes. Consistent with this role, mutations of MeCp2 are linked to Rett syndrome (RTT), a progressive neurological disorder that is one of the most common causes of mental retardation in females.

Immunogen

KLH-conjugated linear peptide corresponding to human MeCp2.

Application

Anti-MeCP2 Antibody is a Chicken Polyclonal Antibody for detection of MeCP2 also known as methyl CpG binding protein 2 (Rett syndrome), mental retardation X-linked 16 & has been validated in WB.

Chromatin Immunoprecipitation Analysis: A representative lot of this antibody was used by an independent laboratory in ChIP. (Thatcher, K., et al. (2005). Hum. Mol. Genet. 14(6): 785-797.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Thatcher, K., et al. (2005). Hum. Mol. Genet. 14(6): 785-797.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Target description

~75 kDa observed

Physical form

Antigen Affinity Purified

Purified chicken polyclonal in buffer with 0.05% sodium azide and 50% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in SH-SY5Y nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected MeCp2 on 10 µg of SH-SY5Y nuclear extract.

Analysis Note

Control
SH-SY5Y nuclear extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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