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ABE1800 Sigma-Aldrich

Anti-La-Related Protein 1

clone, from rabbit, purified by affinity chromatography

Synonym: La ribonucleoprotein domain family member 1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, L-LL, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   human, human, human
shipped in   ambient
packaging   antibody small pack of 25 μg
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_056130
UniProt accession no.   Q6PKG0
Gene Information   human ... LARP1(23367)

Description

General description

La-related protein 1 (UniProt: Q6PKG0; also known as La ribonucleoprotein domain family member 1) is encoded by the LARP1 (also known as KIAA0731, LARP) gene (Gene ID: 23367) in human. La-related protein 1 is a cytoplasmic RNA-binding protein that promotes translation of specific classes of mRNAs downstream of the mTORC1 complex. It contains 1 HTH La-type RNA-binding domain and it associates with the mRNA 5′cap in an mTOR-dependent manner and associates with mRNAs containing a 5′ terminal oligopyrimidine (5′TOP) motif, which is present in mRNAs encoding for ribosomal proteins and several components of the translation machinery. La-related protein 1 is also shown to associates with actively translating ribosomes via interaction with polyadenylate-binding protein 1 (PABPC1/PABP) and stimulates translation of mRNAs containing a 5′TOP, thereby regulating cell growth and proliferation. It is shown to be up-regulated in a number of hepatocellular carcinoma cell lines and liver cancer lesions, as well as in patients with hepatocellular carcinoma with a lower survival rate. (Ref.: Fonseca, BD et al. (2015). J. Biol. Chem. 290(26):15996-6020).

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human La-related protein 1.

Application

Anti-La-Related Protein 1 Antibody, Cat. No. ABE1800, is a highly specific rabbit polyclonal antibody that targets La-related protein 1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected La-Related Protein 1 in HEK293 cells.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected La-Related Protein 1 in human cerebellum, human tonsil, and human pancreas tissues.

Research Category
Epigenetics & Nuclear Function

Target description

~123 kDa observed; 123.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected La-Related Protein 1 in 10 µg of HEK293 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Protocols

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