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ABE1860 Sigma-Aldrich

Anti-CDK13

Anti-CDK13, Cat. No. ABE1860, is a highly specific rabbit polyclonal antibody that targets Cyclin-dependent kinase 13 and has been tested in Western Blotting.

Synonym: CDC2-related protein kinase 5, Cell division cycle 2-like protein kinase 5, Cell division protein kinase 13, Cholinesterase-related cell division controller, Cyclin-dependent kinase 13, hCDK13

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Purified
molecular weight   ~170 kDa observed; 164.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
application(s)   Western Blotting
clone   Polyclonal Antibody
purification method   Affinity Purified
concentration   Please refer to lot specific datasheet.
host   Rabbit
species reactivity   Human
NCBI accession no.   NP_003709
UniProt accession no.   Q14004
gene symbol   CDK13(8621)
  CDC2L5
  CDC2L
  CHED
  KIAA1791
material size   100 µL
shipped in   ambient
storage conditions   Stable for 1 year at 2-8°C from date of receipt.

Description

Background information

Cyclin-dependent kinase 13 (UniProt: Q14004: also known as Cdk13, EC:2.7.11.22, EC:2.7.11.23, CDC2-related protein kinase 5, Cell division cycle 2-like protein kinase 5, Cell division protein kinase 13, hCDK13, Cholinesterase-related cell division controller) is encoded by the CDK13 (also known as CDC2L, CDC2L5, CHED, KIAA1791) gene (Gene ID:8621) in human. CDK13 is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are known to play a vital role as master switches in cell cycle control. Human CDK 13 shares 43% sequence identity with CDK12. CDK13 is expressed in fetal brain, liver, muscle, in adult brain and in neuroblastoma and glioblastoma tumors. It contains expanded regions of serine-arginine (SR) motifs in its N-terminal regions, which links it to the SR protein family involved in RNA processing and pre-mRNA splicing. CDK13 contains a C-terminal extension helix that is composed of a polybasic cluster and a DCHEL motif that interacts with the bound ATP. CDK13 displays C-terminal repeat domain (CTD) kinase activity and is required for RNA splicing. It hyperphosphorylates the C-terminal heptapeptide repeat domain of the largest RNA polymerase II subunit RPB1 and acts a key regulator of transcription elongation. Phosphorylation of the three serine residues (Ser 2, 5, and 7) within the CTD is linked to the phases of RNAPII-mediated transcription. Phosphorylation of Ser7 (pSer7) starts the transcription cycle that primes the CTD for further modifications. (Ref.:Greifenberg, AK et al. (2016). Cell Reports 14(2): 320-331).

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Application

Western Blotting Analysis: A representative lot detected CDK13 in HeLa cell lysate (Bartkowiak, B., et. al. (2010). Genes Dev. 24(20):2303-16).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in HEK293 whole cell extract. (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in HeLa nuclear extract. (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in OVCA5 and HEK293 (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).


Specificity

This polyclonal antibody specifically detects CDK13 in HEK293 cells.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Cyclin-dependent kinase 13 (CDk13).

Species Reactivity Notes

Human. Predicted to react with Bovine based on 100% sequence homology.

Quality Assurance

Evaluated by Western Blotting in HEK293 whole cell extract.

Western Blotting Analysis: 1:500 dilution of this antibody detected CDK13 in 20 µL of HEK293 whole cell extract.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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