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ABE333 Sigma-Aldrich

Anti-MeCP2 Antibody, Isoform B

from rabbit, purified by affinity chromatography

Synonym: Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MB-ME, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   human, human
species reactivity (predicted by homology)   mouse (based on 100% sequence homology), rabbit (based on 100% sequence homology)
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_001104262
UniProt accession no.   P51608-2
Gene Information   human ... MECP2(4204)

Description

General description

DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.

Specificity

Other homologies: Rat (94% sequence homology).

This antibody recognizes isoform B of MeCP2, but does not recognize isoform A. Additionally, this antibody recognizes the N-terminus of isoform B.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human MeCP2.

Application

Detect MeCP2 using this rabbit polyclonal antibody, Anti-MeCP2 Antibody & Isoform B validated for use in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Target description

~75 kDa observed. The calculated molecular weight is 53 kDa, however MeCP2 has been shown as a ~75 kDa band in western blots (Jarrar, M. H., et al. (2003). J Child Neurol. 18(10):675-682)(Zachariah, R., et al. (2012). PLOS. 7(11):1-10).

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected MeCP2 in 10 µg of MCF7 cell lysate.

Analysis Note

Control
MCF7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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