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ABE487 Sigma-Aldrich

Anti-THRAP3 Antibody

from rabbit

Synonym: Thyroid hormone receptor-associated protein 3, Thyroid hormone receptor-associated protein complex 150 kDa component, Trap150

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TF-TJ
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_005110
UniProt accession no.   Q9Y2W1

Description

General description

Thyroid hormone receptor-associated protein 3 (THRAP3) is also called Thyroid hormone receptor-associated protein complex 150 kDa component (Trap150). THRAP3 plays a role in pre-mRNA splicing and is possibly involved in nuclear mRNA decay. THRAP3 regulates signal-induced alternative splicing, cyclin-D1/CCND1 mRNA stability and response to DNA damage. THRP3 is ubiquitously expressed across tissues.

Immunogen

Recombinant protein corresponding to human THRAP3.

Application

Detect Thyroid hormone receptor-associated protein 3 using this rabbit polyclonal antibody, Anti-THRAP3 Antibody validated for use in IP, ICC & western blotting.

Immunoprecipitation Analysis: A representative lot immunoprecipitated THRAP3 in U20S cell lysates (Beli, P., et al. (2012). Mol Cell. 46(2):212-215).
Immunocytochemistry Analysis: A representative lot detected THRAP3 in U20S cells (Bracken, C. P., et al. (2008). Cancer Res. 68(18):7621-7628).
Western Blotting Analysis: A representative lot detected THRAP3 in untreated, and etoposide treated, hydroxyurea treated, 4NQO treated, and irradiated U20S cell lysates (Beli, P., et al. (2012). Mol Cell. 46(2):212-215).
Western Blotting Analysis: A representative lot detected THRAP3 in U20S and THRAP3 transfected HEK293 cell lysates (Bracken, C. P., et al. (2008). Cancer Res. 68(18):7621-7628.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Target description

~150 kDa observed

Physical form

Format: Purified

Protein A/G

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected THRAP3 in 10 µg of U2OS cell lysate.

Analysis Note

Control
U2OS cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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