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ABF210 Sigma-Aldrich

Anti-MDA5 Antibody

Detect Melanoma differentiation-associated protein 5 using this rabbit polyclonal antibody, Anti-MDA5 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Synonym: MDA5 Antibody, Melanoma differentiation-associated protein 5, Interferon-induced with helicase C domain protein 1, IFIH1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µg (ABF210)
application(s)   immunofluorescence: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
biological source   rabbit
clone   polyclonal
purified by   affinity chromatography
shipped in   wet ice
species reactivity   human
gene symbol   IFIH1(64135)
molecular weight   ~117 kDa observed. Uncharacterized bands may appear in some lysates.
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
Quality Assurance   Evaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected MDA5 in 15 µg of Daudi cell lysate.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~117 kDa observed. Uncharacterized bands may appear in some lysates.

Analysis Note

Control
Daudi cell Lysate

Application

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected MDA5 in human lymph node tissue lysate.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected MDA5 in human lymph node cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

General description

Melanoma differentiation-associated protein 5 (MDA5), also known as Interferon-induced helicase C domain-containing protein 1 (IFIH1) is a member of the helicase family (RLR subfamily). MDA5 plays an important role in recognizing various viral components and triggers antiviral responses. When stimulated by dsRNA, MDA5 recruits adaptor protein VISA causing the activation of IRF-3 and NF-κB. MDA5 is widely expressed at low levels with barely detectable levels found in , testis and lung. Mutations in MDA5 have been associated with diabetes mellitus insulin-dependent type 19.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human MDA5.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

This antibody recognizes the C-terminus of MDA5.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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