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ABF222 Sigma-Aldrich

Anti-UHRF1BP1 Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: Ubiquitin-like containing PHD and RING finger domains 1-binding protein 1, ICBP90-binding protein 1, UHRF1 binding protein 1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human, mouse
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
UniProt accession no.   Q6BDS2
Gene Information   human ... UHRF1BP1(54887)

Description

General description

UHRF1-binding protein 1 (UHRF1BP1), also known as ICBP90-binding protein 1 and Ubiquitin-like containing PHD and RING finger domains 1-binding protein 1, is a transcription and cell cycle regulator. UHRF1BP1 is required for proper heterochromatin formation in mammalian cells and may act as a negative regulator of cell growth. UHRF1BP1 is implied to be an important target for the ERK1/2 signaling pathway to control the proliferation of Jurkat T cells. Additionally, UHRF1BP1 binds to the histone H3 N-terminal tail when methylated on K9 by two functional domains, a PHD finger and an SRA (SET- and RING-associated) domain. The PHD finger defines the binding specificity and an SRA domain promotes binding activity.

Immunogen

KLH-conjugated linear peptide corresponding to human UHRF1BP1.

Application

Anti-UHRF1BP1 Antibody is a highly specific rabbit polyclonal antibody, that targets UHRF1-binding protein 1 & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected UHRF1BP1 in human kidney tissue.

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

Target description

~80 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected UHRF1BP1 in 15 µg of mouse kidney tissue lysate.

Analysis Note

Control
Mouse kidney tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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