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ABN1346 Sigma-Aldrich

Anti-SHIP1 Antibody

This Anti-SHIP1 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of SHIP1.

Synonym: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1, hp51CN, Inositol polyphosphate-5-phosphatase of 145 kDa, p150Ship, SH2 domain-containing inositol 5′-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP-1, SHIP1, SIP-145

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SG-SK
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human, human, human
shipped in   wet ice
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_001017915
UniProt accession no.   Q92835

Description

General description

Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (EC 3.1.3.86; UniProt Q92835; also known as hp51CN, Inositol polyphosphate-5-phosphatase of 145 kDa, p150Ship, SH2 domain-containing inositol 5′-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP-1, SIP-145) is encoded by the INPP5D (also known as SHIP, SHIP1) gene (Gene ID 3635) in human. SHIP1 and SHIP2 are related phosphatases that play important roles in modulating cell signaling by regulating the phosphorylation state of phosphatidylinositol phosphates (PIPs). While SHIP1 and SHIP2 share a high degree of amino acid conservation, they differ significantly in their cellular expression and receptor recruitment. SHIP1 is primarily confined to cells of the hematopoietic lineage, although it is also expressed by embryonic and mesenchymal stem cells (ESCs and MSCs). SHIP2 is ubiquitously expressed in all cell and tissue types, with especially high levels found in the heart, skeletal muscle, and placenta. SHIP1 functions as s negative regulator in immunoreceptor signaling, in hematopoietic progenitor cell proliferation/survival, and as an inducer of cellular apoptosis. Humna SHIP1 consists of an N-terminal SH2 domain (a.a. 5-101) that mediates interactions with a large number of intracellular signaling proteins. SHIP1 also contains three SH3-interacting regions (a.a. 124-129, 969-974, 1040-1051) characterized by the presence of the PxxP motifs and two NPXY motifs (a.a. 912-915 & 1019-1022) that interact with SH2 domain-containing proteins.

Specificity

Immunogen sequence is present in all three spliced isoforms of human SHIP1 reported by UniProt (Q92835).

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human SHIP1.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SHIP1 in human lymphoma tissue, as well as in normal tonsil and kidney tissue sections.

Research Category
Neuroscience

Research Sub Category
Kinases & Phosphatases

Target description

~145 kDa observed. Target band size appears larger than the calculated molecular weights of 133.63 kDa (isoform 1), 133.2 kDa (isoform 2), 109.3 kDa (isoform 3) due to posttranslational phosphorylation.

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected SHIP1 in 10 µg of Ramos cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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