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ABN1445 Sigma-Aldrich

Anti-PPAR-gamma Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, PPAR-gamma, PPARgamma

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, PP-PQL, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   human, mouse, human, mouse
species reactivity (predicted by homology)   porcine (based on 100% sequence homology), monkey (based on 100% sequence homology), feline (based on 100% sequence homology), canine (based on 100% sequence homology)
shipped in   ambient
packaging   antibody small pack of 25 μg
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_056953
UniProt accession no.   P37231
Gene Information   human ... PPARG(5468)

Description

General description

Peroxisome proliferator-activated receptor gamma (UniProt P37231; also known as Nuclear receptor subfamily 1 group C member 3, PPAR-gamma, PPARgamma) is encoded by the PPARG (also known as GLM1, NR1C3) gene (Gene ID 5468) in human. The peroxisome proliferator-activated receptors (PPARs) are nuclear receptor proteins that function as transcription factors. PPARs target genes play essential roles in the regulation of cellular differentiation, development, metabolism (carbohydrate, lipid, protein), and tumorigenesis. Three types of PPARs exist, namely alpha, delta (beta), and gamma. In addition, alternative splicings produce three PPAR-gamma isoforms. All PPARs heterodimerize with retinoid X receptor (RXR) and bind peroxisome proliferator hormone response elements (PPREs; AGGTCANAGGTCA) in the promoter region of target genes. PPARγ is activated by prostaglandin PGJ2 and certain members of the 5-HETE family of arachidonic acid metabolites, including 5-oxo-15(S)-HETE and 5-oxo-ETE.

Specificity

This polyclonal antibody targets an internal sequence present in all spliced isoforms of human and murine PPAR-gamma reported by UniProt (P37231 and P37238).

Immunogen

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human PPAR-gamma.

Application

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected PPAR-gamma in HUVECs, A431, HeLa, HEK293, and NIH/3T3 cells..
Western Blotting Analysis: 1 µg/mL from a representative lot detected PPAR-gamma in 10 µg of human skeletal muscle tissue lysate.

This Anti-PPAR-gamma Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PPAR-gamma.

Target description

~55/60 kDa observed. 54.68 kDa (human PPARgamma1(wt); isoform 1), 57.62 kDa (human isoform 2), 21.58 kDa (human PPARgamma1(tr); isoform 3), 54.51 kDa (mouse isoform 1), 57.60 kDa (mouse isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PPAR-gamma in 10 µg of MCF-7 cell lysate.

Linkage

Replaces: 07-466

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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