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  • ABN1737 - Anti-BHLHE40/DEC1 Antibody

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ABN1737 Sigma-Aldrich

Anti-BHLHE40/DEC1 Antibody

serum, clone, from rabbit

Synonym: Class E basic helix-loop-helix protein 40, bHLHe40, Class B basic helix-loop-helix protein 2, bHLHb2, Differentially expressed in chondrocytes protein 1, DEC1, Enhancer-of-split and hairy-related protein 2, SHARP-2, Stimulated by retinoic acid gene 13 pr

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, B-BI, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   human, human
species reactivity (predicted by homology)   rat (based on 100% sequence homology), mouse (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), horse (based on 100% sequence homology)
shipped in   dry ice
antibody form   serum
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_003661
UniProt accession no.   O14503
Gene Information   human ... BHLHE40(8553), DEC1(50514), STRA13(201254)

Description

General description

Class E basic helix-loop-helix protein 40 (UniProt O14503; also known as Basic helix-loop-helix domain containing class B2, Class B basic helix-loop-helix protein 2, Differentially expressed in chondrocytes 1, Differentially expressed in chondrocytes protein 1, Differentiated embryo chondrocyte expressed gene 1, Enhancer-of-split and hairy-related protein 2, SHARP-2, Stimulated by retinoic acid gene 13 protein) is encoded by the BHLHE40 (also known as BHLHB2, DEC1, HLHB2, SHARP2, STRA13) gene (Gene ID 8553) in human. DEC1 (differentially expressed in chondrocytes 1) and DEC2 are E-box-binding transcription factors that exhibit a circadian expression pattern. Both proteins act to repress the Clock/Bmal1-activated E-box promoters via direct E-box binding rather than Bmal1 interaction. In addition, DEC1 is shown to negatively regulate the expression of DEC2. Markedly increased DEC1 expression is observed in colon carcinomas, whereas the opposite trend is seen with DEC2. Likewise cellular DEC1 over-expression results in a proportional decrease in DEC2 level.

Immunogen

Epitope: N-terminal

KLH-conjugated linear peptide corresponding to human BHLHE40/DEC1.

Application

Anti-BHLHE40/DEC1 Antibody is an antibody against BHLHE40/DEC1 for use in Western Blotting.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Target description

~48 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in K562 nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected BHLHE40/DEC1 in 10 µg of K562 nuclear extract.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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