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ABN2242 Sigma-Aldrich

Anti-Vangl2

from rabbit, purified by affinity chromatography

Synonym: Vang-like protein 2 (UniProt: Q9ULK5, also known as Loop-tail protein 1 homolog, Strabismus 1, Van Gogh-like protein 2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, V
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   human, mouse
species reactivity (predicted by homology)   rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
packaging   antibody small pack of 25 μg
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_065068
UniProt accession no.   Q9ULK5
Gene Information   human ... VANGL2(57216)

Description

General description

Vang-like protein 2 (UniProt: Q9ULK5; also known as Loop-tail protein 1 homolog, Strabismus 1, Van Gogh-like protein 2) is encoded by the VANGL2 (also known as KIAA1215, STB1) gene (Gene ID: 57216) in human. Vangl2 is a homodimeric multi-pass membrane protein that plays a role in the control of early morphogenesis, patterning of both axial midline structures and the development of neural plate. It can also form heterodimer with VANGL1. It is required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Vang-like protein 2 is reported to bind to Dvl to cell-autonomously promote efficient Dvl plasma membrane recruitment, a pre-requisite for planar cell polarity activation. At the same time, it can inhibit Dvl from interacting with its downstream effector (Dishevelled associated activator of morphogenesis 1 (Daam1), and functionally suppresses Dvl - Daam1 cascade. Mutations in VANGL2 gene cause neural tube defects that results in defective neural tube closure during the first trimester of pregnancy. (Ref.: Seo, HS et al. (2017). Hum. Mol. Genet. 26(11); 2053-2061).

Specificity

This rabbit polyclonal antibody detects Vang-like protein 2 in human and murine cells. It targets an epitope with in 15 amino acids from the N-terminal region.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Vang-like Protein 2.

Application

Anti-Vangl2, Cat. No. ABN2242, is a highly specific rabbit polyclonal antibody that targets Vang-like protein 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vangl2 in HT1080 cells.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Vangl2 in human tonsil and mouse embryonic spinal cord tissue sections.

Research Category
Neuroscience

Target description

~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Vangl2 in HepG2 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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