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ABN297 Sigma-Aldrich

Anti-Oxytocin receptor Antibody

from rabbit, purified by affinity chromatography

Synonym: Oxytocin receptor, OT-R

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, O, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   human, mouse
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_000907
UniProt accession no.   P30559
Gene Information   human ... OXTR(5021)

Description

General description

Oxytocin is a cyclic 9 amino acid peptide that differs from vasopressin in 2 amino acids. Despite the close similarities in sequence, oxytocin and vasopressin have different biological activities and bind to distinct G protein-coupled receptors. The oxytocin receptor, OT, couples primarily to Gq/11 to mobilize intracellular calcium. In female reproduction, oxytocin promotes uterine contraction and lactation; oxytocin is the most commonly used drug for induction of labor, whereas an oxytocin antagonist, atosiban, is under investigation to suppress preterm labor. Oxytocin/OT interaction in the CNS also plays an important role in stress, male and female sexual response, and sociality.

Immunogen

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Oxytocin receptor.

Application

Anti-Oxytocin receptor Antibody is a highly specific rabbit polyclonal antibody, that targets Oxytocin Receptor & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Target description

~45/65 kDa observed The calculated molecular weight of this protein is 43 kDa However, this protein has been observed at ~60-70 kDa due to glycoslyatation (Zhong, M.m et al. (2012). Molecular Cancer Research. 1-25). Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Oxytocin receptor in 10 µg of MCF7 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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