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ABN407 Sigma-Aldrich

Anti-GLUT9 Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: Solute carrier family 2, facilitated glucose transporter member 9, Glucose transporter type 9, GLUT-9

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, GD-GL, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   rat, human, mouse
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_064425
UniProt accession no.   Q9NRM0
Gene Information   human ... SLC2A9(56606)

Description

General description

GLUT9, also known as Glucose transporter type 9 or GLUT-9, and encoded by the gene SLC2A9/GLUT9, is a member of the sugar transport family that primarily transports urate and fructose; GLUT9 does also transport glucose but at a lower rate than its preferred sugars. GLUT9 also plays a role in urate reabsorption by the proximal tubules of the kidney. GLUT9 is localized to the plasma membrane. GLUT9 is most strongly expressed in basolateral membranes of proximal renal tubular cells, liver and placenta. GLUT9 is also detected in lung, blood leukocytes, heart skeletal muscle and chondrocytes from articular cartilage. Isoform 2 is only detected in the apical membranes of polarized renal tubular cells and placenta. Defects in GLUT9 may be associated with Hypouricemia renal 2 (RHUC2) a disorder characterized by impaired uric acid reabsorption at the apical membrane of proximal renal tubule cells, and high urinary urate excretion.

Specificity

This antibody is expected to recognize both known isoforms of GLUT9.

Immunogen

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human GLUT9.

Application

Detect GLUT9 using this Anti-GLUT9 Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected GLUT9 in human cerebellum and hindbrain tissues.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Target description

~62 kDa observed. Uniprot describes 2 isoforms at 59 kDa (isoform 1) and 55 kDa (isoform 2).

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected GLUT9 in 10 µg of human liver tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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