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ABN482 Sigma-Aldrich

Anti-SYPL1 Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: Synaptophysin-like 1 protein, H-SP1, Pantophysin

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
UniProt accession no.   Q16563
Gene Information   human ... SYPL1(6856)

Description

General description

The synaptophysin-like protein 1, also known as pantophysin, is a homolog of the neuroendocrine-specific protein synaptophysin, with the highest level of homology across its four transmembrane domains. Unlike synaptophysin however, SYPL1 is ubiquitously expressed and found in small cytoplasmic transport vesicles regardless of their content. SYPL1 is thought to play a multifunctional role in vesicle biogenesis and transport and is a component of adipocyte transport vesicles, and thus may be involved in adipocyte secretion. SYPL1 also interacts with vesicle-associated membrane protein 2 (VAMP-2) in adipocytes and associates with GLUT4-containing vesicles.

Immunogen

KLH-conjugated linear peptide corresponding to human SYPL1.

Application

Anti-SYPL1 Antibody is a highly specific rabbit polyclonal antibody, that targets Synaptophysin-like protein 1 & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SYPL1 in human cerebellum, colon, and thalamus tissues.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Target description

~28 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~26 kDa and ~29 kDa

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting/Peptide Inhibition Assay in human brain tissue lysate.

Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected SYPL1 in 15 µg of human brain tissue lysate.

Analysis Note

Control
Human brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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