• USA Home
  • ABS1609 - Anti-Stk25 Antibody

EMAIL THIS PAGE TO A FRIEND
ABS1609 Sigma-Aldrich

Anti-Stk25 Antibody

serum, from rabbit

Synonym: Serine/threonine-protein kinase 25, Ste20-like kinase, Sterile 20/oxidant stress-response kinase 1, SOK-1, Ste20/oxidant stress response kinase1, Stk25

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, ST-ST
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   mouse
species reactivity (predicted by homology)   rat (based on 100% sequence homology)
shipped in   dry ice
antibody form   serum
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_067512
UniProt accession no.   Q9Z2W1

Description

General description

Serine/threonine-protein kinase 25 (EC2.7.11.1; UniProt Q9Z2W1; also known as SOK-1, Ste20-like kinase, Ste20/oxidant stress-response kinase 1, Sterile 20/oxidant stress-response kinase 1, Yeast Sps1/Ste20-related kinase 1) is encoded by the Stk25 gene(Also known as Sok1, Ysk1, C86992, AU018434, 1500019J11Rik) in murine species (Gene ID 59041). STK25, MST3, and MST4 constitute the GCK-III subfamily of the germinal center kinases (GCKs) that are implicated in multiple biological processes, including cell proliferation, apoptosis, migration, polarity, stress responses, and in metabolic and immune regulation. GCK-III kinases are constitutively autophosphorylated with significant basal activities, which can be enhanced several folds upon MO25 binding. GCK-III kinases are components of supramolecular complex called striatin-interacting phophatase and kinase (STRIPAK). The LKB1-Stk25-Golgi matrix protein 130 (GM130) and Reelin-Dab1 pathways operate in opposite directions to provide a proper balance in regulating neuronal polarization and dendritic Golgi deployment.

Immunogen

Epitope: Near C-terminus

GST-tagged recombinant protein corresponding to mouse Stk25 sequence near the C-terminus.

Application

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-Stk25 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of Stk25 .

Western Blotting Analysis: A representative lot detected Stk25 in mouse cortical neurons (Matsuki, T., et al. (2010). Cell. 143(5):826-836).
Immunohistochemistry Analysis: A representative lot detected Stk25 in mouse hippocampal neurons (Matsuki, T., et al. (2010). Cell. 143(5):826-836).

Target description

~52 kDa observed

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in mouse hippocampus tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Stk25 in 10 µg of mouse hippocampus tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?