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ABS1672 Sigma-Aldrich

Anti-SH2B Adapter Protein 1

Detect SH2B adapter protein 1 using this rabbit polyclonal Anti-SH2B Adapter Protein 1 Antibody, Cat. No. ABS1672. It has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Synonym: FceRI gamma-chain-interacting protein SH2-B, SH2 domain-containing protein 1B, SH2-B PH domain-containing signaling mediator

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SG-SK
clone   polyclonal
biological source   rabbit
application(s)   immunofluorescence: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   rat, rat, rat, mouse, mouse, mouse
shipped in   ambient
antibody form   serum
isotype   IgG
antibody product type   primary antibodies
NCBI accession no.   NP_604451
UniProt accession no.   Q62985

Description

General description

SH2B adapter protein 1 (UniProt: Q62985; also known as FceRI gamma-chain-interacting protein SH2-B, SH2 domain-containing protein 1B, SH2-B PH domain-containing signaling mediator 1) is encoded by the Sh2b1 (also known as Sh2-b, Sh2bpsm1) gene (Gene ID: 89817) in rat. SH2B Adapter Protein 1 is an adapter protein for several members of the tyrosine kinase receptor family that is ubiquitously expressed and shuttles between the nucleus and the cytoplasm. It is involved in multiple signaling pathways mediated by Janus kinase (JAK) and receptor tyrosine kinases, including the receptors of insulin, IGF-1, PDGF, FGF, growth hormone, nerve growth factor, brain-derived neurotrophic factor (BDNF), and glial cell line-derived neurotrophic factor (GDNF). It is shown to bind to activated NGF receptor TrkA and has been implicated in NGF-induced neuronal differentiation and the survival of sympathetic neurons. In growth hormone signaling, autophosphorylated (Tyr813) JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. Growth hormone also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin signaling, it binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, SH2 adapter protein 1 binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2.

Specificity

This polyclonal antibody specifically recognizes the beta isoform. It targets an epitope residing within 154 amino acids from the C-terminal half of rat SH2B adapter protein 1.

Immunogen

GST-tagged recombinant fragment corresponding to 154 amino acids from the SH2 domain and C-terminal tail of rat SH2B adapter protein 1.

Application

Research Category
Signaling

Western Blotting Analysis: A representative lot detected SH2B adapter protein 1 in transfected COS cells (Rui, L., et. al. (1997). Mol Cell Biol. 17(11):6633-44).

Immunoprecipitation Analysis: A representative lot detected SH2B adapter protein 1 in COS cells in Immunoprecipitation applications (Rui, L., et. al. (1997). Mol Cell Biol. 17(11):6633-44).

Western Blotting Analysis: A representative lot detected SH2B adapter protein 1 in hippocampal neurons (Chen, C.J., et. al. (2015). J Biol Chem. 290(10):6010-21).

Immunofluorescence Analysis: A representative lot detected SH2B adapter protein 1 in hippocampal neurons (Chen, C.J., et. al. (2015). J Biol Chem. 290(10):6010-21).

Target description

~80 kDa observed; 79.64 kDa calculated Uncharacterized bands may be observed in some lysate(s).

Physical form

Rabbit polyclonal antiserum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:500 dlution of this antibody detected SH2B adapter protein 1 in 10 µg of PC12 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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