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ABS1673 Sigma-Aldrich

Anti-Uteroglobin

serum, clone, from goat

Synonym: Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Blastokinin

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   polyclonal
biological source   goat
application(s)   immunofluorescence: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   mouse, mouse
species reactivity (predicted by homology)   rat (based on 100% sequence homology)
shipped in   ambient
antibody form   serum
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_037183
UniProt accession no.   P17559

Description

General description

Uteroglobin (UniProt: P17559; also known as Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Blastokinin) is encoded by the Scgb1a1 (also known as Cc10, Ugb, Utg) gene (Gene ID: 25575) in rat. Uteroglobin, is a member of the Secretoglobin superfamily that serves as a multifunctional protein and can exert anti-inflammatory and anti-tumorigenic effects by binding small hydrophobic molecules. Uteroglobin is synthesized with a signal peptide of 19 amino acids and consists of a disulfide-linked dimer of two identical polypeptides, each polypeptide being composed of four helices. Uteroglobin derives its name from its high levels of expression in pre-implantation embryos, where it exhibits growth stimulatory effects. It is produced and secreted by the non-ciliated, non-mucous Clara cells predominant in the epithelial surfaces of pulmonary airways. It plays a role in sequestering pro-inflammatory mediators and carcinogens and has been implicated in the inhibition of cell migration and invasion. It inhibits soluble phospholipase A2 activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, phospholipids, prostaglandins, and retinols. Uteroglobin positive (Scgb1a1+) cells in different regions of the lung differ in their role as progenitors in vivo. For example, in the bronchioles, these cells can both self-renew and generate ciliated cells during postnatal growth and adult homeostasis. On the other hand, in the trachea, the majority of uteroglobin positive cells constitute a transient amplifying population during postnatal growth and steady state, and their capacity for self-renewal and multi-lineage differentiation increases in response to injury. (Ref.: Mukherjee, AB et al. (2007). Endocrine Rev. 28(7): 707-725).

Specificity

This polyclonal antibody detects uteroglobin in mouse and rat tissues.

Immunogen

Epitope: unknown

His-tagged full length recombinant rat uteroglobin.

Application

Detect Uteroglobin using this goat polyclonal Anti-Uteroglobin Antibody, Cat. No. ABS1673 that has been tested in Immunofluorescence, immunohistochemistry, and Western blotting.

Immunofluorescence Analysis: A representative lot detected Uteroglobin in dedifferentiated cells (Tata, P.R., et. al. (2013). Nature. 503(7475):218-23) and mice E18.5 lung tissue (Hashimoto, S., et. al. (2012). J Cell Sci. 125(Pt 4):932-42).

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Uteroglobin in mouse lung tissue. (Courtesy of Dr.Barry Stripp at Cedars-Sinai Medical Center, Los Angeles, CA).

Immunohistochemistry Analysis: A representative lot detected Uteroglobin in adult mice alveoli (Rawlins, E.L., et. al. (2009). Cell Stem Cell. 4(6):525-34).

Research Category
Signaling

Target description

~10 kDa observed; 10.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Goat polyclonal antiserum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Uteroglobin in 10 µg of mouse lung tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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