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ABS434 Sigma-Aldrich

Anti-ApoA4 Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: Apolipoprotein A4, Apolipoprotein A-IV, ApoA-IV, APO-AIV, ApoA4

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories AO-AQ, Alphabetical Index, Antibodies, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
UniProt accession no.   P06727
Gene Information   human ... APOA4(337)

Description

General description

ApoA4, also known as Apolipoprotein A4 or ApoA-IV, is a member of the apolipoprotein A1/A4/E family. ApoA4 is a major component of HDL and it can be found on the surface of newly synthesized chylomicrons. Studies have shown that APoA4 is required for efficient activation of lipoprotein lipase by ApoC-II, which is a potent activator of Lecithin Cholesterol Acyltransferase (LCAT). Other evidence points out that ApoA4 may have a role in chylomicrons and VLDL secretion and catabolism. The physiological function of ApoA4 is not confirmed yet, but evidence from several in vitro studies indicate that it may play a role in the reverse cholesterol transport pathway, transporting cholesterol from peripheral cells back to the liver. ApoA4 is synthesized primarily in the intestine and secreted in the plasma.

Immunogen

KLH-conjugated linear peptide corresponding to human ApoA4.

Application

Anti-ApoA4 Antibody is a highly specific rabbit polyclonal antibody, that targets Apolipoprotein A & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected ApoA4 in human colon tissue.

Research Category
Signaling

Research Sub Category
Lipid Metabolism & Weight Regulation

Target description

~38 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected ApoA4 in 15 µg of human liver tissue lysate.

Analysis Note

Control
Human liver tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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