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ABS459 Sigma-Aldrich

Anti-USP10 Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10, Ubiquitin thioesterase 10, Ubiquitin-specific-processing protease 10, UPS10

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
UniProt accession no.   Q14694

Description

General description

Ubiquitin carboxyl-terminal hydrolase 10 (USP10), also known as Ubiquitin-specific-processing protease 10, belongs to the peptidase C19 family and functions to catalyze the cleavage of ubiquitin from ubiquitin-conjugated protein substrates such as p53/TP53, SNX3 and CFTR. USP10 is widely expressed and is down-regulated in renal cell carcinomas.

Immunogen

KLH-conjugated linear peptide corresponding to human USP10.

Application

Anti-USP10 Antibody is a highly specific rabbit polyclonal antibody, that targets Ubiquitin carboxyl-terminal hydrolase 10 & has been tested in western blotting & ICC.

Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected USP10 in Jurkat cells.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Target description

~122 kDa observed. Uniprot describes 3 isoforms at ~~87 kDa, ~88 kDa, and ~93 kDa

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected USP10 in 15 µg of Jurkat cell lysate.

Analysis Note

Control
Jurkat cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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