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ABS498 Sigma-Aldrich

Anti-GOLPH3 Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: Golgi phosphoprotein 3, Coat protein GPP34, MIDAS, Mitochondrial DNA absence factor

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, GM-GO, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   human, human
species reactivity (predicted by homology)   mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_071413
UniProt accession no.   Q9H4A6
Gene Information   human ... GOLPH3(64083)

Description

General description

Golgi phosphoprotein 3 (UniProt Q9H4A6; also known as Coat protein GPP34, MIDAS, Mitochondrial DNA absence factor) is encoded by the GOLPH3 (also known as GPP34) gene (Gene ID 64083) in human. GOLPH3 is a phosphatidylinositol 4-phosphate- (PI4P-) binding protein that plays an essential role in vesicle trafficking and Golgi structure. GOLPH3 is localized to the trans-Golgi via tight binding to PI4P that is highly enriched at the trans-Golgi. GOLPH3 interacts and recruits the unconventional myosin MYO18A to the Golgi, allowing MYO18A and F-actin interaction. The complex applies a tensile force that pulls and deforms the Golgi membrane to facilitate vesicle budding and subsequent vesicles trafficking from the Golgi to the plasma membrane. GOLPH3/MYO18A pathway is reported to mediate DNA-PK-dependent DNA damage response that is essential for normal cell survival and, when enhanced, can confer resistance to killing by DNA damaging agents. GOLPH3 is found overexpressed in many solid tumors and cancer cell lines. Overexpression of GOLPH3 occurs frequently in rhabdomyosarcoma and GOLPH3 knockdown impairs the proliferation of rhabdomyosarcoma cell lines.

Specificity

This polyclonal antibody stains Golgi apparatus by indirect fluorescence immunocytochemistry. This polyclonal antibody specifically detected the target band in lysate from control, but not GOLPH siRNA-transfected HeLa cells.

Immunogen

Epitope: Near N-terminus.

KLH-conjugated linear peptide corresponding to an N-terminal region sequence of human GOLPH3.

Application

Anti-GOLPH3 Antibody is an antibody against GOLPH3 for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected GOLPH3 in HeLa cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GOLPH3 in 10 µg of lysate from untransfected, but not GOLPH siRNA-transfected, HeLa cells (Courtesy of Matthew Buschman, Ph.D. and Seth Field, M.D., Ph.D., University of California, San Diego, USA).

Research Category
Signaling

Target description

~34 observed. 33.81 kDa calculated.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected GOLPH3 in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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