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  • ABS79 - Anti-phospho-mTOR (Ser2159) Antibody

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ABS79 Sigma-Aldrich

Anti-phospho-mTOR (Ser2159) Antibody

Synonym: Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapam

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MS-MUD, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   dot blot: suitable
  inhibition assay: suitable (peptide)
  western blot: suitable
species reactivity   rat, human, human, sheep, mouse, zebrafish
species reactivity (predicted by homology)   mouse (based on 100% sequence homology), rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), sheep (based on 100% sequence homology)
shipped in   wet ice
antibody product type   primary antibodies
Quality Level   100
purified by   affinity chromatography
NCBI accession no.   NP_004949
UniProt accession no.   P42345
Gene Information   human ... MTOR(2475)

Description

General description

The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.

Specificity

This antibody recognizes mTOR phosphorylated at Ser2159.

Immunogen

Epitope: Phosphorylated at Ser2159

KLH-conjugated linear peptide corresponding to human mTOR phosphorylated at Ser2159.

Application

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Western Blot Analysis: A representative lot detected phospho-mTOR (Ser2159) in HEK293 cell lysate. Image courtesy from the laboratory of Dr. Diane Fingar, University of Michigan Medical School.

Target description

290 kDa calculated

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Dot Blot in unmodified and modified mTOR peptides.

Dot Blot Specificity Analysis: Unmodified and modified mTOR peptides (see Table) were probed with Anti-phospho-mTOR (Ser2159) (1:1,000 dilution).

Analysis Note

Control
Unmodified and modified mTOR peptides

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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