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  • ABS88 - Anti-phospho-mTOR (Thr2164) Antibody

ABS88 Sigma-Aldrich

Anti-phospho-mTOR (Thr2164) Antibody

Synonym: Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapam

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, MS-MUD, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   dot blot: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
antibody form   serum
antibody product type   primary antibodies
Quality Level   100
NCBI accession no.   NP_004949
UniProt accession no.   P42345
Gene Information   human ... MTOR(2475)


General description

The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.


This antibody recognizes mTOR phosphorylated at Thr2164.


Epitope: Phosphorylated Thr2164

KLH-conjugated linear peptide corresponding to human mTOR phosphorylated at Thr2164.


Research Category

Research Sub Category
Kinases & Phosphatases

Western Blot Analysis: A representative lot was used by an independent laboratory in HEK293 cell lysate. (Ekim, B., et al. (2011). MCB. [Epub ahead of print].)

Target description

289 kDa calculated

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.


Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Dot Blot Specificity Analysis: Unmodified and phosphorylated mTOR peptides were probed with Anti-phospho-mTOR (Thr2164) (1:1,000 dilution).

Analysis Note

Unmodified and phosphorylated mTOR peptides

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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