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ABT176 Sigma-Aldrich

Anti-Protocadherin-10 Antibody

Detect Protocadherin using this rabbit polyclonal antibody, Anti-Protocadherin-10 Antibody validated for use in western blotting & IHC (Paraffin).

Synonym: Protocadherin-10

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
gene symbol   PCDH10(57575)
NCBI accession no.   NP_116586
UniProt accession no.   Q9P2E7
Quality Assurance   Evlauted by Western Blotting in HEK293 cell lysates.

Western Blotting Analysis: 2 µg/mL of this antibody detected Protocadherin-10 in 10 µg of HEK293 cell lysates.
Species Reactivity Notes   Demonstrated to react with Human. Predicted to react with Mouse, Bovine based on 100% sequence homology.
application(s)   Western Blotting, Immunohistochemistry (Paraffin)
clone   Polyclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
format   Affinity Purified
host   Rabbit
molecular weight   ~130 kDa observed. The calculated molecular weight of this protein is 113 kDa. However, Uniprot states that this protein is glycosylated, which may result in higher molecular weights. Uncharacterized band(s) may be observed in some cell lysates.
packaging   100 µg (ABT1760)
  100 μg (ABT1760)
shipped in   wet ice
species reactivity   Human

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
HEK293 cell lysate

Application

Detect Protocadherin using this rabbit polyclonal antibody, Anti-Protocadherin-10 Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Protocadherin-10 in human cerebellum, thalamus, and testis tissues.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

General description

Protocadherin-10 is a Single-pass type I membrane protein that is likely involved in calcium-dependent cell-adhesion. Protocadherin-10 is moderately expressed in brain testis and ovaries, with low expression in most other tissues.

Immunogen

Epitope: Cadherin 6 domain

KLH-conjugated linear peptide corresponding to the Cadherin 6 domain of human Protocadherin-10.

Specificity

This antibody recognizes the Cadherin 6 domain of Protocadherin-10.

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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