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  • ABT256 - Anti-Telo-Collagen Type I, A1/COL1A1

ABT256 Sigma-Aldrich

Anti-Telo-Collagen Type I, A1/COL1A1

clone, from rabbit, purified by affinity chromatography

Synonym: Collagen alpha-1(I) chain, Alpha-1 type I collagen

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TC-TE
clone   polyclonal
biological source   rabbit
application(s)   flow cytometry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   sheep, human, mouse, sheep, human, mouse
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_000079
UniProt accession no.   P02452
Gene Information   human ... COL1A1(1277)


General description

Collagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends.


Cat. No. ABT256 Anti-Telo-Collagen Type I, A1/COL1A1 was raised against a sequence derived from the C-terminal telopeptide (not the N-terminal telopeptide) region. This polyclonal antibody will detect prepro-, pro-, and mature collagen alpha-1(I) chain, but not atelocollagen prepared with proteolytic enzymes due to the absence of the C-terminal telopeptide sequence.

Predicted to react with a broad range of species based on high sequence homology, including mammalian, avian, and amphibian species.


Epitope: C-terminal telopeptide region

Linear peptide corresponding to sequence derived from human Collagen Type I, A1/COL1A1 C-terminal telopeptide region.


Anti-Telo-Collagen Type I Antibody, A1/COL1A1 is an antibody against Telo-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western Blotting Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung and skin tissue lysate.
Flow Cytometry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.
Immunohistochemistry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.

Target description

~180 kDa observed

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Telo-Collagen Type I, A1/COL1A1 in 1 µg of mouse liver tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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