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  • AC111 - Sheep Anti-Mouse IgG Antibody, Species absorbed: Rat IgG

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AC111 Sigma-Aldrich

Sheep Anti-Mouse IgG Antibody, Species absorbed: Rat IgG

clone, from sheep

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Mouse Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies,
Quality Level   100
biological source   sheep
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   mouse, mouse
application(s)   ELISA: suitable
shipped in   wet ice

Description

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Based on ELISA analysis, this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reaction mouse IgA or IgM.

This antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with Human IgG, Rabbit IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG, but may cross-react with immunoglobulins from other species.

Immunogen

Purified antibody corresponding to mouse IgG.

Application

ELISA (Isotype) Analysis: A representative lot of this antibody detected whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reaction mouse IgA or IgM.

Research Category
Secondary & Control Antibodies

Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Sheep Anti-Mouse IgG Antibody, Species absorbed: Rat IgG is an antibody against Sheep Mouse IgG for use in ELISA.

Quality

Evaluated by ELISA using IgG antibodies from various species.

ELISA Analysis: This antibody detected 200 ng of mouse IgG antibody among a panel of IgG antibodies from various species.

Polysorp® is a registered trademark of Thermo Fisher Scientific.

Target description

150 kDa calculated

Physical form

Format: Purified

Fractionated by Octanoic Acid and Ammonium Sulfate cut and then depleted with Rat IgG column and Mouse IgA column.

Purified sheep Polyclonal serum in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

PolySorp is a registered trademark of Thermo Fisher Scientific or its subsidiaries

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Peer-Reviewed Papers
15

References

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