• USA Home
  • AP112A - Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate

EMAIL THIS PAGE TO A FRIEND
AP112A Sigma-Aldrich

Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate

clone, Chemicon®, from goat

  •  eCl@ss 32160702

  •  NACRES NA.46

Purchase

Properties

Related Categories Alkaline Phosphatase, Antibodies, Human IgG Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   human
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  western blot: suitable
conjugate   alkaline phosphatase conjugate
shipped in   wet ice

Description

General description

Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Human IgG (H+L)

Specificity

Based on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species.

Application

Detect Human IgG using this Goat anti-Human IgG Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.

EIA and Western blots: 1:5,000-1:50,000.

Immunohistochemistry: 1:1,000-1:2,000.

Optimal working dilutions must be determined by end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Linkage

Replaces: MABN1055

Physical form

Lyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with

15 mg/mL BSA and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

Serum

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade for better) to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

For research use only; not for use as a diagnostic.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
WGK Germany 
WGK 3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?