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AP112P Sigma-Aldrich

Goat Anti-Human IgG Antibody, HRP conjugate

clone, Chemicon®, from goat

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Human IgG Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody form   affinity isolated antibody
antibody product type   secondary antibodies
clone   polyclonal
form   lyophilized
species reactivity   human
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  western blot: suitable
isotype   IgG
conjugate   peroxidase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It is a glycoprotein and mainly helps in immune defense. About 70 percent of the total immunoglobulin consists of IgG. IgG is a monomeric immunoglobulin, made up of four polypeptide chains. It consists of two 50 kDa heavy chains (gamma) and two 25kDa light chains. These chains are inter-linked by di-sulfide bonds. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Specificity

The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.

Immunogen

Prepared from purified human IgG.

Application

Goat Anti-Human IgG Antibody, HRP conjugate has been used:
• to detect the bound immunoglobulin G (IgG) in enzyme-linked immunosorbent assay (ELISA) assay
• in immunohistochemistry or cytochemistry at a dilution of 1:500 - 1:5,000
• for western blotting with chromogenic substrates at a dilution of 1:5,000 - 1:100,000 and with ECL substrates at a dilution of 1:10,000 - 1:200,000.
• for EIA at a dilution of 1:5,000-1:100,000

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It initiates the classical pathway of the complement system and also neutralizes virus particles and toxins. Maternal IgG can pass through the human placenta to fetus providing immune defense for the neonate against infections. It facilitates antibody-dependent cell-mediated cytotoxicity (ADCC). IgG is digested by papain to produce fragment antigen-binding (Fab). Pepsin digestion of IgG results in the formation of fragment crystallizable (Fc). IgG levels in severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patients may be useful to evaluate the severity and prognosis of coronavirus disease 2019 (COVID-19).

Physical form

Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.

Storage and Stability

Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.

WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Other Notes

Research Category: Secondary & Control Antibodies

Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Secondary Antibodies, Conjugates and Kits

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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