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AP112P Sigma-Aldrich

Goat Anti-Human IgG Antibody, HRP conjugate

Detect Human IgG using this Goat anti-Human IgG Antibody, HRP conjugate validated for use in ELISA & WB.

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Human IgG Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   human
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  western blot: suitable
conjugate   peroxidase conjugate
shipped in   wet ice

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. About 70 percent of the total immunoglobulin consists of IgG.

Specificity

The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.

Immunogen

Prepared from purified human IgG.

Application

Goat Anti-Human IgG Antibody, HRP conjugate has been used to detect the bound immunoglobulin G (IgG) in enzyme-linked immunosorbent assay (ELISA) assay. It is suitable for use in immunohistochemistry.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Physical form

ImmunoAffinity Purified

Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.

Storage and Stability

Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.

WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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