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  • AP113F - Goat Anti-Human IgG Antibody, Fc, FITC conjugate

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AP113F Sigma-Aldrich

Goat Anti-Human IgG Antibody, Fc, FITC conjugate

clone, 2 mg/mL, Chemicon®

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, FITC Antibodies, Human IgG Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   human
mfr. no.   Chemicon®
concentration   2 mg/mL
application(s)   immunofluorescence: suitable
conjugate   FITC conjugate
shipped in   wet ice

Description

Specificity

Shows a single band when run in an IEP system at a minimum concentration of 10 mg/mL, using human whole serum as the antigen. Reacts with Human IgG Fc fragment. No reactivity to other human immunoglobulins is observed.

Application

Goat anti-Human IgG Antibody, Fc, FITC conjugate is an antibody against Human IgG for use in IF.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

Suggested dilution for most applications: 1:50-1:200

Optimal working dilutions must be determined by the end user.

Physical form

PRESENTATION: Liquid 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA, 0.1% sodium azide.

FLUOROPHORE/PROTEIN:

12.0 μg/mg

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months. DO NOT FREEZE. Store in the dark.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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