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AP124F Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, (H+L) FITC Conjugated

Goat anti-Mouse IgG Antibody, (H+L) FITC Conjugated is an antibody against Mouse IgG for use in IF.

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Properties

Related Categories Antibodies, Secondary Antibodies More...
trade name   Chemicon
brand family   Chemicon
format   FITC
application(s)   Immunofluorescence
clone   Polyclonal Antibody
purification method   ImmunoAffinity Purified
concentration   2 mg/mL (after reconstitution)
host   Goat
species reactivity   Mouse
material size   2 mg
shipped in   wet ice
storage conditions   Maintain lyophilized product at 2-8°C for up to 12 months after date of receipt. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Protect from light.

Description

Background information

Immunoglobulin G (IgG) is a multimeric immunoglobulin, built of two heavy chains γ and two light chains. Each complex has two antigen binding sites. It is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. Furthermore, this is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Physical form

Lyophilized from 0.02 M phosphate buffered saline, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide and 15 mg/mL bovine serum albumin.
F/P Ratio:
14.5 μg FITC per mg of antibody

Application

Affinity purified antibody shows a single band against Rabbit anti-Goat Serum when run as the antigen in IEP at a minimum concentration of 10 mg/mL.

Suggested dilution for most applications: 1:50-1:200.

Optimal working dilutions must be determined by the end user.

Specificity

Affinity purified antibody shows a single band when run in an IEP system at a minimum concentration of 10 mg/mL using whole mouse serum as the antigen.
Purity:
Affinity purified antibody shows a single band against Rabbit anti Goat Serum when run as the antigen in IEP at a minimum concentration of 10 mg/mL.

Quality Assurance

Affinity purified antibody shows a single band against Rabbit anti Goat Serum when run as the antigen in IEP at a minimum concentration of 10 mg/mL.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Peer-Reviewed Papers
15

References

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