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  • AP132P - Goat Anti-Rabbit IgG Antibody, Peroxidase Conjugated

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AP132P Sigma-Aldrich

Goat Anti-Rabbit IgG Antibody, Peroxidase Conjugated

clone, 1 mg/mL (after reconstitution), Chemicon®

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Peroxidase Labeled Antibodies, Rabbit Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   rabbit
mfr. no.   Chemicon®
concentration   1 mg/mL (after reconstitution)
application(s)   ELISA: suitable
  immunohistochemistry: suitable
  western blot: suitable
isotype   IgG
conjugate   peroxidase conjugate
shipped in   wet ice

Description

General description

Affinity purified goat anti-rabbit, HRP conjugated.

Specificity

Specific for rabbit IgG, heavy and light chain.

Application

ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.

Immunohistochemistry:
1:500-1:5,000 dilution can be used.

Optimal working dilutions must be determined by end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Whole Immunoglobulin Secondary Antibodies

This Goat anti-Rabbit IgG Antibody, Peroxidase Conjugated is validated for use in ELISA, IH, WB for the detection of Rabbit IgG.

Physical form

ImmunoAffinity Purified

Purified goat IgG conjugated to horseradish peroxidase in buffer containing 0.01 M Phosphate Buffered Saline, pH 7.1 with 15 mg/mL BSA and 0.01% Thimerosal. Lyophilized.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months from date of shipment.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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