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  • AP147B - Rabbit Anti-Sheep IgG Antibody, biotin-SP conjugate

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AP147B Sigma-Aldrich

Rabbit Anti-Sheep IgG Antibody, biotin-SP conjugate

Chemicon®, from rabbit

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Biotin Antibodies, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies,
Quality Level   100
biological source   rabbit
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   sheep
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  western blot: suitable
conjugate   biotin conjugate
shipped in   wet ice

Description

Specificity

Based on immunoelectrophoresis, the antibody reacts with the heavy chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum protein, but the antibody may cross-react with immunoglobulins from other species.

Immunogen

Purified Sheep IgG.

Application

DILUTION:

1:50,000 - 1:1,000,000 for ELISA using enzyme-conjugated streptavidin

1:10,000 - 1:100,000 for Western Blots using enzyme-conjugated streptavidin

1:500 - 1:5,000 for enzyme immunohistochemistry on tissue sections

1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry

Optimal working dilution must be determined by the end user.

Rabbit anti-Sheep IgG Antibody, biotin-SP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA & WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Physical form

ImmunoAffinity Purified

Lyophilized. Buffer = 0.01M Phosphate buffer, 0.25 M NaCl with 15 mg/mL BSA and 0.05% sodium azide.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months from date of receipt. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Expiration date: one year from date of reconstitution.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Hazard statements 
Precautionary statements 
WGK Germany 
WGK 3
Protocols & Articles

Articles

Antibody Basics

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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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