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AP175 Sigma-Aldrich

Goat Anti-Human IgE Antibody

clone, 3.1 mg/mL, Chemicon®

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Human IgE Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   human
mfr. no.   Chemicon®
concentration   3.1 mg/mL
application(s)   ELISA: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
conjugate   unconjugated
shipped in   dry ice

Description

Specificity

Human IgE. Single arc by IEP against IgE elevated serum. Less than 1% reactivity with IgM, IgG, IgD and IgA.

Immunogen

Human myeloma IgE

Application

Goat anti-Human IgE Antibody is an antibody against Human IgE for use in ELISA, IP & WB.

Physical form

Immunoglobulin, affinity purified. Liquid in 0.05M Tris-HCl (pH 7.5), 0.5M NaCl, 0.01M EDTA with 0.1% sodium azide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

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Peer-Reviewed Papers
15

References

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