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AP180F Sigma-Aldrich

Donkey Anti-Goat IgG Antibody, FITC conjugate, Species Adsorbed

Detect Donkey Goat IgG using this Donkey anti-Goat IgG Antibody, FITC conjugate, Species Adsorbed validated for use in IF.

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, FITC Antibodies, Goat Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
biological source   donkey
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   goat
mfr. no.   Chemicon®
application(s)   immunofluorescence: suitable
conjugate   FITC conjugate
shipped in   wet ice

Description

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Goat IgG

WAVELENGTH: Absorption peak = 492 nm, Emission peak = 520 nm.

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.5% sodium azide.

RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.

FLUOROPHORE/PROTEIN:
Approximately 8.2 μg/mg.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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