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AP187F Sigma-Aldrich

Goat Anti-Rabbit IgG Antibody, FITC conjugate, Species Adsorbed

Chemicon®, from goat

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, FITC Antibodies, Rabbit Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   rabbit
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  immunofluorescence: suitable
isotype   IgG
conjugate   FITC conjugate
shipped in   wet ice

Description

General description

IgG is one of the most abundant protein in human serum. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen.

Specificity

The antibody reacts with whole molecule rabbit IgG. It also reacts with the llight chains of other rabbit immunoglobulins. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with human, mouse and rat immunoglobulins.

Application

Goat anti-Rabbit IgG Antibody, FITC conjugate, Species Adsorbed detects level of Rabbit IgG & has been published & validated for use in ELISA & IF.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Physical form

Immunoaffinity Chromatography

Purified from immunoaffinity chromatography. Lyophilized from 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6, 15 mg/ml Bovine Serum Albumin (Protease-Free, IgG-Free) and 0.05 Sodium Azide.

Storage and Stability

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 1 ml water will yield a final concentration of 1.5 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Hazard statements 
Precautionary statements 
WGK Germany 
WGK 3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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