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AP192F Sigma-Aldrich

Donkey Anti-Mouse IgG Antibody, FITC conjugate, Species Adsorbed

Detect Donkey Mouse IgG using this Donkey anti-Mouse IgG Antibody, FITC conjugate, Species Adsorbed validated for use in IF.

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Properties

Related Categories Antibodies, Secondary Antibodies More...
trade name   Chemicon
brand family   Chemicon
format   FITC
application(s)   Immunofluorescence
clone   Polyclonal Antibody
purification method   ImmunoAffinity Purified
host   Donkey
species reactivity   Mouse
material size   500 µg
shipped in   wet ice
storage conditions   Maintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Description

Background information

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Physical form

Lyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6, with 15 mg/mL BSA and 0.1% sodium azide.

RECONSTITUTION:

Reconstitute to 1 mg/mL with sterile distilled water.

F/P RATIO:

15 micrograms FITC per mg of antibody.

Application

Suggested dilution for most applications: 1:50-1:200.

Optimal working dilutions must be determined by the end user.

Specificity

Specific for Mouse IgG.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Peer-Reviewed Papers
15

References

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