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AP202 Sigma-Aldrich

Goat Anti-Rat light chain Antibody

clone, Chemicon®, from goat

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Rat Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   rat
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  western blot: suitable
isotype   IgG
conjugate   unconjugated
shipped in   wet ice

Description

General description

Light chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain′s mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Specificity

Minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep.

The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep immunoglobulins.

Immunogen

Epitope: Kappa light chain

Prepared from rat IgG light chain.

Application

Goat anti-Rat light chain Antibody is an antibody against light chain for use in ELISA & WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

Western Blotting

ELISA

Optimal working dilutions must be determined by the end user.

Target description

25 kDa

Physical form

ImmunoAffinity Purified

Purified by immunoaffinity chromatography. Liquid in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6.

Storage and Stability

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

Antibody Basics

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The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Protocols

Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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