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  • AP501A - Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed

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AP501A Sigma-Aldrich

Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed

Chemicon®, from goat

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Alkaline Phosphatase, Antibodies, Mouse Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   mouse
mfr. no.   Chemicon®
application(s)   ELISA: suitable
conjugate   alkaline phosphatase conjugate
shipped in   dry ice

Description

General description

IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Specificity

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.

Application

ELISA: 1:2,000-1:4,000

Optimal working dilutions must be determined by the end user.

Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed detects level of Mouse IgM+IgG+IgA & has been published & validated for use in ELISA.

Physical form

Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Store long term at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

Antibody Basics

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The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Secondary Antibodies, Conjugates and Kits

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Peer-Reviewed Papers
15

References

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