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  • AP501F - Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, FITC conjugate, Species Adsorbed

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AP501F Sigma-Aldrich

Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, FITC conjugate, Species Adsorbed

Chemicon®, from goat

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, FITC Antibodies, Mouse Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   mouse
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  immunofluorescence: suitable
conjugate   FITC conjugate
shipped in   wet ice

Description

General description

IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Specificity

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.

The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Immunogen

Epitope: Mouse IgM, IgG, IgA

Prepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.

Application

Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, FITC conjugate, Species Adsorbed is an antibody against Mouse IgM+IgG+IgA (H+L) for use in ELISA & IF.

Immunofluorescence: ≤ 1 µg/106 cells
ELISA
Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Physical form

Affinity Chromatography

Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 1.0 mL PBS/NaN3.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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