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AP504A Sigma-Aldrich

Goat Anti-Human IgG γ chain Antibody, Alkaline Phosphatase conjugate, Species Adsorbed

clone, Chemicon®, from goat

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Alkaline Phosphatase, Antibodies, Human IgG Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   human
mfr. no.   Chemicon®
application(s)   ELISA: suitable
conjugate   alkaline phosphatase conjugate
shipped in   wet ice

Description

General description

IgG is one of the most abundant protein in human serum. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Reacts with with the heavy chain of human IgG. Absorbed for human IgM, IgD and IgA.

The antibody reacts with the heavy chain of human IgG as demonstrated by ELISA and flow cytometry.

Immunogen

Epitope: IgG γ chain

Prepared from IgG γ chain. Pooled antisera from goats hyperimmunized with normal human IgG and human IgG paraproteins.

Application

Detect Human IgG γ chain using this Goat anti-Human IgG γ chain Antibody, Alkaline Phosphatase conjugate, Species Adsorbed validated for use in ELISA.

ELISA: 1:2,000-1:4,000.

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Fragment Specific Secondary Antibodies

Physical form

Affinity Chromatography

Purified by affinity chromatography on pooled human IgG covalently linked to agarose. Liquid in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

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