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AP504B Sigma-Aldrich

Goat Anti-Human IgG γ chain Antibody, biotin conjugate, Species Adsorbed

clone, Chemicon®, from goat

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Biotin Antibodies, Human IgG Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   human
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  immunofluorescence: suitable
conjugate   biotin conjugate
shipped in   wet ice

Description

General description

IgG is one of the most abundant protein in human serum. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Reacts with with the heavy chain of human IgG. Absorbed for human IgM, IgD and IgA.

The antibody reacts with the heavy chain of human IgG as demonstrated by ELISA and flow cytometry.

Immunogen

Epitope: IgG γ chain

Prepared from IgG γ chain. Pooled antisera from goats hyperimmunized with normal human IgG and human IgG paraproteins.

Application

Immunofluorescence: ≤1 μg/106 cells.
ELISA: 1:5,000-1:20,000.

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Fragment Specific Secondary Antibodies

This Goat anti-Human IgG γ chain Antibody, biotin conjugate, Species Adsorbed is validated for use in ELISA, IF for the detection of Human IgG γ chain.

Physical form

Affinity Chromatography

Purified by affinity chromatography on pooled human IgG covalently linked to agarose. Liquid in 1.0 mL PBS/NaN3.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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