• USA Home
  • AP510 - Sheep-Anti-Rabbit IgG secondary Antibody

AP510 Sigma-Aldrich

Sheep-Anti-Rabbit IgG secondary Antibody

clone, 2 mg/mL, Chemicon®

  •  eCl@ss 32160702

  •  NACRES NA.46



Related Categories Antibodies, Rabbit Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies,
Quality Level   100
biological source   sheep
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   rabbit
mfr. no.   Chemicon®
concentration   2 mg/mL
application(s)   ELISA: suitable
shipped in   wet ice


General description

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.


This antibody reacts with rabbit IgG and rabbit IgG (Fc) as demonstrated by ELISA. Minimal cross-reactivity detected with Human IgG and Rabbit IgM; little to no cross-reactivity detected with Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.


Rabbit IgG


Research Category
Secondary & Control Antibodies

Research Sub Category
Isotype Control Antibodies

Sheep-anti-Rabbit IgG secondary antibody detects level of Rabbit IgG secondary & has been published & validated for use in ELISA.


Evaluated by ELISA against Human IgG, Mouse IgG, Rat IgG, Chicken IgM, Goat IgG, Guinea Pig IgG, Hamster IgG, Rabbit IgG, Rabbit IgG (Fc), and Rabbit IgM.

ELISA Analysis: A representative lot detected Sheep Anti-Human IgM, (Fc) in ELISA. (1:200 - 1,000 recommended dilution.)

Target description

150 kDa calculated

Physical form

Format: Purified

Octanoic Acid and Ammonium Sulfate cut. Depleted against Goat IgG and Rabbit IgM

Purified sheep polyclonal in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Human IgG, Mouse IgG, Rat IgG whole molecule, Chicken IgM whole molecule, Goat IgG whole molecule, Guinea Pig IgG, Hamster IgG whole molecule, Rabbit IgG, Rabbit IgG (Fc), and Rabbit IgM whole molecule

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?