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  • AQ112P - Goat Anti-Human IgG Antibody, F(ab′)2, HRP conjugate

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AQ112P Sigma-Aldrich

Goat Anti-Human IgG Antibody, F(ab′)2, HRP conjugate

clone, Chemicon®, from goat

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Human IgG Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   human
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  western blot: suitable
conjugate   peroxidase conjugate
shipped in   wet ice

Description

Application

Goat anti-Human IgG Antibody, F(ab′)2, HRP conjugate detects level of Human IgG & has been published & validated for use in ELISA & WB.

Storage and Stability

Reconstitution: Store freeze-dried product at 4°C until opened for up to 12 months. Reconstitute to 1 mg/mL with distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. (To judge clarity, draw product into a pasteur pipette). Product is stable for several weeks at 4°C as an undiluted liquid. Discard dilutions after more than one day. For extended storage after reconsitutiton, add an equal volume of glycerol to make a final concentation of 50% glycerol and store at -20°C. Please remember that the concentration of protein (and buffer salts) will decrease to one-half of the original after addition of glycerol.

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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