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  • AQ160 - Rabbit Anti-Mouse IgG Antibody, F(ab′)2

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AQ160 Sigma-Aldrich

Rabbit Anti-Mouse IgG Antibody, F(ab′)2

clone, 1 mg/mL, Chemicon®

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Mouse Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies,
Quality Level   100
biological source   rabbit
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   mouse
mfr. no.   Chemicon®
concentration   1 mg/mL
application(s)   ELISA: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
conjugate   unconjugated
shipped in   wet ice

Description

Specificity

Reacts with mouse IgG gamma chain, as well as the light chains from all mouse immunoglobulin classes. Monospecific by IEP against whole mouse serum.

Immunogen

Highly purified mouse IgG isolated from pooled normal mouse serum.

Application

Optimal working dilutions must be determined by end user.

Rabbit anti-Mouse IgG Antibody, F(ab′)2 is an antibody against Mouse IgG for use in ELISA, IP & WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

Quality

Pepsin digest shows a single band on IEP at an antigen concentration of 10 mg/mL when run against anti-mouse IgG (H&L) and anti-F(ab′)2. No reaction was observed against anti-rabbit IgG (Fc).

Physical form

Affinity purified immunoglobulin, F(ab′)2 fragment. Liquid in 0.02 M Phosphate Buffer, 0.25 M NaCl, 0.1% sodium azide.

Storage and Stability

Store at +2-8°C for up to six months.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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