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AQ303F Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, F(ab′)2, FITC conjugate

1 mg/mL, Chemicon®

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, FITC Antibodies, Mouse Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   goat
antibody product type   secondary antibodies
clone   polyclonal
species reactivity   mouse
mfr. no.   Chemicon®
concentration   1 mg/mL
application(s)   immunofluorescence: suitable
conjugate   FITC conjugate
shipped in   wet ice

Description

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

The reagent is an anti-mouse antibody F(ab′)2 fragment from goat. The F(ab′)2 fragments are obtained by pepsin cleavage. The fragments are conjugated to fluorescein isothiocyanate and stabilized in buffer. The molar ratio of fluorescein: protein is: 3 - 7.

PURIFICATION: The goat-lgG was purified by affinity chromatography and absorbed to remove cross-reactivity to human immunoglobulins.

Specificity

Specific for mouse IgG, heavy and light chain. The cross-reactivities of the anti-mouse IgG antibody were tested in an ELISA. Minimum cross-reactivity to human IgG.

Application

Immunohistochemistry: 1:200-1:500 (Coligan et al. 1997; Harlow & Lane 1988; Bullock & Petrusz 1982; Javois 1994)

Immunocytochemistry: 1:200-1:500 (Coligan et al. 1997; Harlow & Lane 1988; Bullock & Petrusz 1982; Javois 1994)

Flow cytometery: 1 μg per 1 x 10E6 cells (Coligan et al. 1997; Harlow & Lane 1988; Javois 1994) FITC absorption peak is 488-492nm, its emission peak is 520nm.

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

This Goat anti-Mouse IgG Antibody, F(ab′)2, FITC conjugate is validated for use in IF for the detection of Mouse IgG.

Physical form

ImmunoAffinity Purified

Liquid in 0.02M Phosphate Buffer. 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA and 0.01% Sodium azide.

Storage and Stability

The antibody conjugate solution is stable at 2–8°C for 12 months. Do not store in a diluted format.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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