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  • CBL1358 - Anti-Endoglin Antibody, Extracellular, clone MJ7/18

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CBL1358 Sigma-Aldrich

Anti-Endoglin Antibody, Extracellular, clone MJ7/18

This Anti-Endoglin Antibody, Extracellular, clone MJ7/18 is validated for use in FC, IP, WB, IH for the detection of Endoglin.

Synonym: CD105, Ancillary TGF-beta Receptor

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, EJ-EN, Primary Antibodies
clone   MJ7/18, monoclonal
biological source   rat
application(s)   flow cytometry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse
shipped in   wet ice
isotype   IgG2aκ
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
suitability   not suitable for immunohistochemistry (Paraffin)
NCBI accession no.   NM_000118.1
UniProt accession no.   P17813

Description

General description

Endoglin is the regulatory component of the TGF-beta receptor complex. CD105 is a homodimer of 90 kDa subunits and is predominantly expressed on vascular endothelial cells. It is also found on pre-erythroblasts, macrophages and lymphoid and myeloid leukemic cells High levels of mouse endoglin mRNA have been detected in ovary, uterus, and NCTC-2071 fibroblasts, and to a lesser extent, in heart and muscle. In addition, stromal cells in the connective tissue of various organs are also positive for endoglin expression. CD105 has been reported to have functions in adhesion and embryonic angiogenesis, and that MJ7/18 is a pan endothelial marker (with minimal reactivity with early hematopoetic cells). Both mouse and human CD105 exhibit >70% sequence similarity in their cytoplasmic domains with type III transforming growth factor β (TGFβ) receptor.

Specificity

Specifically recognizes mouse CD105, known as endoglin.

Immunogen

Inflamed mouse skin (Ge, AZ et al, 1994).

Application

Flow Cytometry: 1μg/ 10(e6) cells in 100 μL total volume.

Immunohistochemical staining of fresh frozen or acetone fixed tissue specimens.1:10-1:50. Not recommended for formalin fixed paraffin tissues.

Immunoprecipitation: Use rabbit anti-rat secondary antibody followed by protein A, or anti-rat beads for recovery.

Western Blotting: 1:500-1:1000. Recognizes a 90 kDa band under reducing conditions. Membrane preparations are recommended. Mouse vascular endothelial cells recommended as a positive control.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Physical form

Format: Purified

Protein A purified immunoglobulin presented as a liquid in 100 mM borate buffered saline, containing no preservatives.

Storage and Stability

Maintain at 2-8°C for up to 12 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
POSITIVE CONTROL: mouse vascular endothelial cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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