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CBL154 Sigma-Aldrich

Anti-H-CAM Antibody, clone F10-44-2

This Anti-H-CAM Antibody, clone F10-44-2 is validated for use in FC, IP, IH, IH(P) for the detection of H-CAM.

Synonym: CD44

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Properties

Related Categories Antibodies, Primary Antibodies More...
trade name   Chemicon
brand family   Chemicon
format   Purified
control   POSITIVE CONTROL: Tonsil; stains all T-cells in the paracortex
application(s)   Flow Cytometry
  Immunoprecipitation
  Immunohistochemistry
  Immunohistochemistry (Paraffin)
isotype   IgG2a
clone   Monoclonal Antibody
  F10-44-2
host   Mouse
species reactivity   Human
NCBI accession no.   NM_000610.3, NM_001001389.1, NM_001001390.1, NM_001001391.1, NM_001001392.1
UniProt accession no.   P16070
gene symbol   CD44(960)
  CD44R
  CDW44
  CSPG8
  ECMR-III
  EPICAN
  HCELL
  HUTCH-I
  IN
  LHR
  MC56
  MDU2
  MDU3
  MGC10468
  MIC4
  MUTCH-I
  PGP-1
  PGP1
material size   100 µg
shipped in   wet ice
storage conditions   For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Description

Physical form

The monoclonal is presented at a concentration of 100μg/mL in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Application

Identification of CD44 positive cells by flow cytometry and immunocytochemistry, in particular with formalin fixed paraffin wax embedded sections of lymphoid and epithelial tissues (high temperature antigen retrieval in 10mM citrate buffer, pH6.0, is recommended).

Studies involving T, B cell, monocyte, granulocyte T and B cell binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites.

Studies on HCAM (homing receptor) function.

Immunoprecipitation.

Optimal working dilutions must be determined by the end user.

Specificity

The CD44 antigen is a transmembranous glycoprotein of 80 kDa, which has undergone both extensive O-linked and N-linked glycosylation. This antibody is specific for epitope 1. The CD44 antigen is present on T lymphocytes, granulocytes, red blood cells, brain and epithelial cells. There is weak expression on platelets.
FUSION PARTNER: NS1 myeloma cell line

Immunogen

Purified human T cells

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (c...
Anna Abai, Nick Asbrock and Vi Chu1
1 MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Clinical, Drug discovery, Gene expression, Immunostaining

Mesenchymal Stem Cell FAQs

Mesenchymal stem cells (MSCs) are defined as a self-renewing population of adherent multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages including bo...
Keywords: Anti-inflammatory agents, Cell disruption, Clinical, Digestions, Diseases, Gene expression, Growth factors, Neurodegenerative Diseases

Protocols

Mesenchymal Stem Cell Culture Protocols

Introduction Methods   Isolation of Mesenchymal Stem Cells   Expansion of Human Mesenchymal Stem Cells     Preparation of Coated Plates     Thawing of Mesenchymal Stem Cells     Expansion of Mesenchy...
Keywords: Cell disruption, Clinical, Culture media, Gene expression, Immunocytochemistry, Stem cell biology

Peer-Reviewed Papers
15

References

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