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CC076 Sigma-Aldrich

Human Collagen Type IV

  •  eCl@ss 32160405

  •  NACRES NA.75



Quality Level   100
biological source   human
assay   95% (Human collagen type IV, SDS-PAGE)
form   liquid
mfr. no.   Chemicon®
concentration   1 mg/mL
application(s)   cell culture | mammalian: suitable
impurities   <5% Human collagen types I-III, V, VI, and non-collagen proteins
input   sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
solubility   water: soluble at 20 °C
NCBI accession no.   NM_001845.4
UniProt accession no.   P02462
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... COL4A1(1282)


General description

Collagen type IV is the major structural element of basal membranes. Human collagen type IV is a native triple helix. Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type IV was purified by using differential salt precipitation. Purity and retention of native helical structure was controlled by SDS-PAGE and by reaction with anti-collagen type specific monoclonal antibodies. Molecular weight: 300kDa; Solubility: 10mg/mL

Product Source: Human placenta, negative for HBsAg and HIV antibodies


Coating material for cell culture studies

Formation of collagen gels

Optimal working dilutions must be determined by end user.

Physical form

Liquid purified protein. In 100 μL of 0.5M acetic acid solution, pH 2.5. Salt-free, no preservative.

Storage and Stability

Store at -20ºC for up to 12 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Retention of native structure was confirmed by ability to form microfibrils.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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