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CC076 Sigma-Aldrich

Human Collagen Type IV

  •  eCl@ss 32160405

  •  NACRES NA.75

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Properties

Quality Level   100
biological source   human
assay   95% (Human collagen type IV, SDS-PAGE)
form   liquid
mfr. no.   Chemicon®
concentration   1 mg/mL
application(s)   cell culture | mammalian: suitable
impurities   <5% Human collagen types I-III, V, VI, and non-collagen proteins
input   sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
solubility   water: soluble at 20 °C
NCBI accession no.   NM_001845.4
UniProt accession no.   P02462
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... COL4A1(1282)

Description

General description

Collagen type IV is the major structural element of basal membranes. Human collagen type IV is a native triple helix. Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type IV was purified by using differential salt precipitation. Purity and retention of native helical structure was controlled by SDS-PAGE and by reaction with anti-collagen type specific monoclonal antibodies. Molecular weight: 300kDa; Solubility: 10mg/mL

Product Source: Human placenta, negative for HBsAg and HIV antibodies

Application

Coating material for cell culture studies

Formation of collagen gels

Optimal working dilutions must be determined by end user.

Physical form

Liquid purified protein. In 100 μL of 0.5M acetic acid solution, pH 2.5. Salt-free, no preservative.

Storage and Stability

Store at -20ºC for up to 12 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Retention of native structure was confirmed by ability to form microfibrils.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

Protocols

ECM Coating Protocols for Millicell

Coating of membranes and plastic surfaces with extra cellular matrices (ECMs) promotes cell attachment and monolayer formation. We have developed protocols for four types of ECMs on Millicell®-CM ins...
Keywords: Cell attachment, Cell culture, Condensations

Peer-Reviewed Papers
15

References

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