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CP11L Sigma-Aldrich

Anti-Integrin α₃ Mouse mAb (P1B5)

lyophilized, clone P1B5, Calbiochem®

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Properties

Related Categories Alphabetical Index, Antibodies, IN-IP, Primary Antibodies
clone   P1B5, monoclonal
biological source   mouse
species reactivity   human
form   lyophilized
shipped in   ambient
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C

Description

General description

Purified mouse monoclonal antibody generated by immunizing RBF/Dn mice with the specified immunogen and fusing splenocytes with NS-1/FOX-NY mouse myeloma cells (see application references). Recognizes the α3 integrin subunit.

Recognizes the α3-subunit of the α31 integrin complex protein in human mononuclear cells.

This Anti-Integrin α₃ Mouse mAb (P1B5) is validated for use in Cell Inhibition Assays, FC, IF, IP for the detection of Integrin α₃.

Immunogen

Human

human mononuclear cells

Application

Cell Inhibition Assays (1-5 µg/ml, see application references)

Flow Cytometry (1-5 µg/ml)

Immunoblotting (not recommended)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg/sample)

Paraffin Sections (not recommended)

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

100 μg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
A2058 cells

Other Notes

Albelda, S.M. and Buck, C.A. 1990. FASEB J.4, 2868.
Cheresh, D.A. et al. 1990. Cell57, 59.
Krissansen, G.W. et al. 1990. J. Biol. Chem.265, 823.
Vogel, B, et al. 1990. J. Biol. Chem.265, 5934.
Wayner, E.A. et al.1988 J. Cell Biol.107, 1881.
Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol.3, 179.
Hynes, R.O. 1987 Cell48, 549.
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.

Will partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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