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CP35 Sigma-Aldrich

Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8)

liquid, clone 7E3-1B8, Calbiochem®

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Properties

Related Categories Alphabetical Index, Antibodies, PS-PS, Primary Antibodies
clone   7E3-1B8, monoclonal
biological source   mouse
species reactivity   rat
form   liquid
shipped in   wet ice
isotype   IgG2a
Quality Level   100
antibody product type   primary antibodies
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C

Description

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with HL-1 mouse myeloma cells (see application references). Recognizes the ~95 kDa PSD-95 protein.

Recognizes the ~95 kDa PSD95 protein.

This Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of PSD-95 (Ab-2).

Immunogen

Rat

recombinant, rat PSD95

Application


Immunoblotting (1:2000)

Immunofluorescence (1:200, indirect)

Immunoprecipitation (see comments)

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Packaging

100 μL in Glass bottle

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Other Notes

Brakeman, P.R., et al. 1997. Nature386, 284.
Hualing, D., et al. 1997. Nature386, 279.
Kennedy, M.B. 1997. Trends in Neurosci.20, 264.
Sanes, J. 1997. Curr. Opin. Neurobiol.7, 93.
Cho., K.-O., et al. 1992. Neuron9, 929.

Immunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95′s proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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